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	Provedor de dados BABT (1) Autor Pereira,Albanin Aparecida Mielniczki (1) Pileggi,Marcos (1) Pileggi,Sônia Alvim Veiga (1) Silva,Joandrei dos Santos (1) Verma,Desh Pal S. (1) Palavra-chave Cold resistance (1) Lettuce (1) Proline (1) Stress resistance (1) Transgenic (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2001 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última An improved method for transformation of lettuce by Agrobacterium tumefaciens with a gene that confers freezing resistance BABT Pileggi,Marcos; Pereira,Albanin Aparecida Mielniczki; Silva,Joandrei dos Santos; Pileggi,Sônia Alvim Veiga; Verma,Desh Pal S.. An efficient method for constructing transgenic lettuce cultivars by Agrobacterium tumefaciens was described by Torres et al., 1993. In the present work, an improvement of the above procedure is described and applied to transform the cultivar Grand Rapids with a mutated P5CS gene. The major modifications were concerned with turning more practical the transformation and regeneration protocols. Also we tried to improve transformation steps by increasing injured area in explants and prolonging co-cultivation with Agrobacteria (in larger concentration). A more significant selective pressure was used against non-transformed plants and bacteria. In these work we were concerned to obtain T1 and T2 seeds. The P5CS gene codes for a delta¹-pyrroline-5-carboxylate... Tipo: Info:eu-repo/semantics/article Palavras-chave: Stress resistance; Cold resistance; Transgenic; Lettuce; Proline. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200013 Registros recuperados: 1 Primeira ... 1 ... Última

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